About the GC Content Calculator
Analyze GC content, base composition, and melting temperature from any DNA/RNA sequence.
What it is
The GC Content Calculator computes the percentage of guanine and cytosine bases in a DNA or RNA sequence, along with full base composition. It accepts raw sequence text or FASTA-formatted input, auto-detects DNA vs RNA, and separately reports any ambiguous IUPAC bases (like N) rather than folding them into the GC% denominator.
Features
FASTA support
Header lines are stripped automatically; multiple sequences get per-sequence stats.
Sliding-window analysis
A configurable window/step chart reveals GC-rich or GC-poor regions.
Melting temperature
Wallace rule for short sequences, GC%-based formula for longer ones.
Validation
Ambiguous bases are counted separately; empty input is blocked.